Biomedsearch home advanced search tools search tutorial login | create free account document detail alpinetin suppresses proliferation of human hepatoma cells by the activation of mkk7 and elevates sensitization to cis-diammined dichloridoplatium. buying viagra pills online
Medline citation: pmid:â 22159816 â â â owner:â nlm â â â status:â publisher â â â abstract/otherabstract: alpinetin is a type of novel plant flavonoid derived from alpinia katsumadai hayata, found to possess strong anti-hepatoma effects.
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what is the best between viagra und viagra und viagra However, the detailed antitumor mechanism of alpinetin remains unclear.
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Mitogen-activated protein kinase kinase-7 (mkk7) can regulate cellular growth, differentiation and apoptosis.
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The aim of this study was to investigate the role of mkk7 in the anti-hepatoma effect mediated by alpinetin.
Hepg2 cells were treated with alpinetin at various doses and for different times, and the levels of phosphorylated mkk7 (p-mkk7) and total mkk7 were tested by rt-pcr and western blotting.
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Following transient transfection with rna interference, cell viability and cell cycle stage were determined using methyl thiazolyl tetrazolium assay and flow cytometry, in order to assess the antitumor action of alpinetin.
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In addition, chemosensitization to cis-diammined dichloridoplatium (cddp) by alpinetin was assessed by cell counting array and the cell growth inhibitory rate was calculated. how many mg of viagra can you take The results showed that alpinetin suppressed hepg2 cell proliferation and arrested cells in the g0/g1 phase by up-regulating the expression levels of p-mkk7.
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On the contrary, inhibiting the expression of mkk7 reversed the antitumor effect of alpinetin. buy cheap viagra in australia Moreover, alpinetin enhanced the sensitivity of hepg2 hepatoma cells to the chemotherapeutic agent cddp. viagra ukrainian techno band Taken together, our studies indicate that activation of mkk7 mediates the anti-hepatoma effect of alpinetin.
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Mkk7 may be a putative target for molecular therapy against hepatoma and alpinetin could serve as a potential agent for the development of hepatoma therapy. viagra generic uk Authors: bo tang; jian du; jingwen wang; guang tan; zhenming gao; zhongyu wang; liming wang related documents : 2104636â -â adherence of uropathogenic e.
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